Disk Diffusion-Based Methods for Determining Candida parapsilosis Susceptibility to Anidulafungin
نویسندگان
چکیده
منابع مشابه
Disk diffusion-based methods for determining Candida parapsilosis susceptibility to anidulafungin.
Zone diameters for anidulafungin by disk diffusion for 139 isolates of C. parapsilosis were compared with MICs by NCCLS M27-A2 broth microdilution. The comparison was poor unless the disks were prepared by dissolving anidulafungin in 1% dimethyl sulfoxide plus 0.1% Tween 80 and testing on Mueller-Hinton agar flooded with glucose and methylene blue.
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A 25-microg fluconazole disk diffusion test using a Mueller-Hinton agar plate containing 2% glucose and 5 microg of methylene blue/ml (GM-MH) was compared to the macrodilution reference method for 210 Candida species. The GM-MH agar plate was read at 24 h. The predictive values of disks with susceptible, intermediate, and resistant results on the GM-MH agar plate at 24 h were 97.1, 56.3, and 76...
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OBJECTIVES To investigate the activities of two newer triazoles and two echinocandins combined with human phagocytes against Candida parapsilosis biofilms. METHODS An in vitro model of C. parapsilosis biofilms was used. Biofilms were grown on silicone elastomer discs in 96-well plates at 37°C for 72 h. Biofilms or planktonic cells were incubated with voriconazole, posaconazole, caspofungin or...
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INTRODUCTION Antifungal susceptibility testing assists in finding the appropriate treatment for fungal infections, which are increasingly common. However, such testing is not very widespread. There are several existing methods, and the correlation between such methods was evaluated in this study. METHODS The susceptibility to fluconazole of 35 strains of Candida sp. isolated from blood cultur...
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ژورنال
عنوان ژورنال: Antimicrobial Agents and Chemotherapy
سال: 2003
ISSN: 0066-4804,1098-6596
DOI: 10.1128/aac.47.9.3018-3020.2003